Volume 528, Issue 1, 12 July 2020, Pages 174-178
Author links open overlay panelJunpeiTakahashia1DaisukeYamadaa1YudaiUetaaTakashiIwaibEriKogaaMitsuoTanabebJun-IchiroOkaaAkiyoshiSaitoha
aLaboratory of Pharmacology, Department of Pharmacy, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan
bLaboratory of Pharmacology, School of Pharmaceutical Sciences, Kitasato University, 5-9-1, Shirokane, Minato-ku, Tokyo, 108-8641, Japan
Received 25 March 2020, Accepted 12 April 2020, Available online 29 May 2020.
•Aβ25–35 decreased hippocampal LTP.
•Oxytocin reversed the Aβ25–35–induced impairment of hippocampal LTP.
•ERK phosphorylation is involved in the effect of oxytocin on LTP.
•Ca2+-permeable AMPA receptors are involved in the effect of oxytocin on LTP.
Oxytocin, a peptide hormone synthesized in the hypothalamic paraventricular nucleus, has been reported to participate in the regulation of learning and memory performance. However, no report has demonstrated the effect of oxytocin on the amyloid-beta (Aβ)-induced impairment of synaptic plasticity. In this study, we examined the effects of oxytocin on the Aβ-induced impairment of synaptic plasticity in mice.
To investigate the effect of oxytocin on synaptic plasticity, we prepared acute hippocampal slices for extracellular recording and assessed long-term potentiation (LTP) with perfusion of the Aβ active fragment (Aβ25–35) in the absence and presence of oxytocin.
We found that oxytocin reversed the impairment of LTP induced by Aβ25–35 perfusion in the mouse hippocampus. These effects were blocked by pretreatment with the selective oxytocin receptor antagonist L-368,899. Furthermore, the treatment with the ERK inhibitor U0126 and selective Ca2+-permeable AMPA receptor antagonist NASPM completely antagonized the effects of oxytocin.
This is the first report to demonstrate that oxytocin could reverse the effects of Aβ on hippocampal LTP in mice. We propose that ERK phosphorylation and Ca2+-permeable AMPA receptors are involved in this effect of oxytocin.